The Gadsden flag, proposing a rattlesnake and the word "Don't Tread on Me

https://deanrshx384.summitviewdaily.com/posts/flags-of-honor-the-symbolism-behind-civil-war-colors-and-emblems

The Gadsden flag, presenting a rattlesnake and the word "Don't Tread on Me," was followed via a few Confederate sets as a image of resistance opposed to perceived oppression.